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SRX130007: GSM896813: 2-cell #3-C1; Homo sapiens; RNA-Seq
1 ILLUMINA (Illumina HiSeq 2000) run: 48.7M spots, 4.9G bases, 3Gb downloads

Submitted by: Gene Expression Omnibus (GEO)
Study: Tracing pluripotency of human early embryos and embryonic stem cells by single cell RNA-seq
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Find the casual relationship between gene expression network and cellular phenotype at single cell resolution. We collected donated human pre-implatation embryos, and the embryonic stem cells derived from them, isolate individual cells, prepared single cell cDNAs, and sequenced them by HiSeq2000. Then we analyzed the expression of known RefSeq genes. Overall design: We get transcriptome of 124 individual cells from human pre-implantation embryos and human embryonic stem cells by applying single cell RNA-seq technique we recently developed[1][2][3][4]. We did in-depth bioinformatic analysis to these data and found very dynamic expression of protein-coding genes. [1] Tang, F. et al. (2010a) Tracing the Derivation of Embryonic Stem Cells from the Inner Cell Mass by Single-Cell RNA-Seq Analysis. Cell Stem Cell 6, 468-478. [2] Tang, F. et al. (2010b) RNA-Seq analysis to capture the transcriptome landscape of a single cell. Nat Protocols 5, 516-535. [3] Tang, F. et al. (2009) mRNA-Seq whole-transcriptome analysis of a single cell. Nat Meth 6, 377-382. [4] Tang, F. et al. (2011) Development and applications of single-cell transcriptome analysis. Nat Meth 8, S6-S11.
Sample: 2-cell embryo#3 -Cell#1
SAMN00828840 • SRS300741 • All experiments • All runs
Organism: Homo sapiens
Library:
Name: GSM896813: 2-cell #3-C1
Instrument: Illumina HiSeq 2000
Strategy: RNA-Seq
Source: TRANSCRIPTOMIC
Selection: cDNA
Layout: SINGLE
Spot descriptor:
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Experiment attributes:
GEO Accession: GSM896813
Links:
External link:
Runs: 1 run, 48.7M spots, 4.9G bases, 3Gb
Run# of Spots# of BasesSizePublished
SRR44572848,727,1884.9G3Gb2013-08-12

ID:
148128

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