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SRX2148931: GSM2306477: EGKX_S6; Gallus gallus; RNA-Seq
1 ILLUMINA (NextSeq 500) run: 78M spots, 23.6G bases, 10.4Gb downloads

Submitted by: NCBI (GEO)
Study: Developmental programs in chicken early embryos by whole transcriptome analysis.
show Abstracthide Abstract
Temporal regulation of gene expression is essential for early embryonic development. With the development of next-generation sequencing technologies, transcriptional profiling of early developmental processes in various vertebrates are already revealed. However, overall transcriptome analysis of early embryonic development in avian have never been investigated. In this study, to our knowledge, we provide comprehensive profiling of pre-oviposited embryos in chicken by whole-transcriptome RNA-seq for the first time. We demonstrated the presence of avian-specific mechanism of first- and second-wave of zygotic genome activation (ZGA). Chicken early embryo have distinct developmental programs between cleavage and area pellucida formation period. Additional speculation of epigenetic dynamics indicate that maternal-to-zygotic transition (MZT) in chicken is promoted after second-wave of ZGA, through EGK.VI to EGK.X stages. These extensive results contribute to understanding avian early embryogenesis and comparative studies with other species with many developmental aspects. Overall design: Chicken early embryo (Oocyte to EGK.X) samples were investigated based on the whole transcriptome sequencing approach.
Sample: EGKX_S6
SAMN05752694 • SRS1679758 • All experiments • All runs
Organism: Gallus gallus
Library:
Instrument: NextSeq 500
Strategy: RNA-Seq
Source: TRANSCRIPTOMIC
Selection: cDNA
Layout: PAIRED
Construction protocol: Total RNA was prepared from the samples and Total RNA was extracted using TRIzol reagent (MRC). Libraries were prepared based on Illimina instruction
Experiment attributes:
GEO Accession: GSM2306477
Links:
Runs: 1 run, 78M spots, 23.6G bases, 10.4Gb
Run# of Spots# of BasesSizePublished
SRR420495877,997,03823.6G10.4Gb2017-12-15

ID:
3143711

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