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SRX1713059: GSM2127195: WT.Epid.NoUV_2; Mus musculus; miRNA-Seq
1 ILLUMINA (Illumina HiSeq 2500) run: 14.8M spots, 753.3M bases, 415.7Mb downloads

Submitted by: NCBI (GEO)
Study: Identification of a novel PPARß/d / miR-21-3p axis in UV-induced skin inflammation [human miRNA-seq]
show Abstracthide Abstract
Although excessive exposure to UV is widely recognized as a major factor leading to skin perturbations and cancer, the complex mechanisms underlying inflammatory skin disorders resulting from UV exposure remain incompletely characterized. The nuclear hormone receptor PPARß/d is known to control cutaneous repair and UV-induced cancer development. Here, we describe a novel PPARß/d-dependent molecular cascade involving TGFß-1 and miR-21-3p, which is activated in the epidermis in response to UV exposure. We establish that the passenger miRNA miR-21-3p, that we identify as a novel UV-induced miRNA in the epidermis, plays a pro-inflammatory function in keratinocytes, and that its high level of expression in human skin is associated with psoriasis and squamous cell carcinomas. Finally, we provide evidence that inhibition of miR-21-3p reduces UV-induced cutaneous inflammation in ex vivo human skin biopsies, thereby underlining the clinical relevance of miRNA-based topical therapies for cutaneous disorders. Overall design: Epidermis and dermis, PPARB KO vs WT, 3-4 biological replicates per condition
Sample: WT.Epid.NoUV_2
SAMN04869822 • SRS1402353 • All experiments • All runs
Organism: Mus musculus
Instrument: Illumina HiSeq 2500
Strategy: miRNA-Seq
Selection: size fractionation
Layout: SINGLE
Construction protocol: Total RNA was isolated using Trizol (Invitrogen). RNA quantities and quality were assessed using a NanoDrop ND-1000 spectrophotometer or an Agilent 2100 BioanalyzerRNA quantities and quality were assessed using a NanoDrop ND-1000 spectrophotometer or an Agilent 2100 Bioanalyzer. Small RNA libraries were prepared using 1 μg of total RNA according to the TruSeq Small RNA Sample Preparation Guide (Illumina, San Diego, CA). Libraries were sequenced HiSeq 2500 using v3 chemistry.
Experiment attributes:
GEO Accession: GSM2127195
Runs: 1 run, 14.8M spots, 753.3M bases, 415.7Mb
Run# of Spots# of BasesSizePublished


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