Instrument: Illumina HiSeq 2500
Strategy: RNA-Seq
Source: TRANSCRIPTOMIC
Selection: cDNA
Layout: PAIRED
Construction protocol: RNA was extracted from frozen embryos using phenol/chloroform and LiCl precipitation and purified by using the Qiagen RNeasy Plant Mini kit. RNA quality and integrity analysis was performed by the Virginia Bioinformatics Institute (Virginia Tech, Blacksburg, VA). Reverse transcription of mRNA, library preparation, and paired-end RNA-Seq generating 75-bp reads, including removing low quality reads and adapter sequence trimming, was performed by Beckman Coulter Genomics (Danvers, MA) using established protocols.