GSM1901342: 47 yo ctx endo; Homo sapiens; RNA-Seq - SRA

SRX1303821: GSM1901342: 47 yo ctx endo; Homo sapiens; RNA-Seq
1 ILLUMINA (NextSeq 500) run: 18.3M spots, 5.5G bases, 2.4Gb downloads

Submitted by: Gene Expression Omnibus (GEO)

Study: RNA-Seq of human astrocytes

PRJNA297760 • SRP064454 • All experiments • All runs

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Astrocytes were purified from fetal and adult human brain tissue using an immunopanning method with the HepaCAM antibody. Samples were taken from otherwise 'healthy' pieces of tissue, unless otherwise specified. Overall design: 6 fetal astrocyte samples, 12 adult astrocyte samples, 8 GBM or sclerotic hippocampal samples, 4 whole human cortex samples, 4 adult mouse astrocyte samples, and 11 human samples of other purified CNS cell types

Sample: 47 yo ctx endo

SAMN04145539 • SRS1098712 • All experiments • All runs

Organism: Homo sapiens

Library:

Instrument: NextSeq 500

Strategy: RNA-Seq

Source: TRANSCRIPTOMIC

Selection: cDNA

Layout: PAIRED

Construction protocol: Tissue was dissociated in papain (30units / ml) for 1.5 hours before trituration. Cells were immunopanned on a sequence of plates in the following order: CD45 --> GalC --> GalC --> Thy1 --> HepaCAM. Cells bound to HepaCAM plate were then scraped in Qiazol to harvest RNA Nugen Ovation V2 kit (7102-32), followed by sonication in the Covaris machine, followed by indexing with NEB Next Ultra kit (E7530L)

Experiment attributes:

GEO Accession: GSM1901342

Links:

NCBI link: NCBI Entrez (gds)

Runs: 1 run, 18.3M spots, 5.5G bases, 2.4Gb

Run	# of Spots	# of Bases	Size	Published
SRR2557122	18,276,004	5.5G	2.4Gb	2015-12-11

ID:: 1861461

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