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SRX1177122: GSM1865851: C2C12_control; Mus musculus; RNA-Seq
1 ILLUMINA (Illumina HiSeq 2000) run: 26.3M spots, 5.3G bases, 3.3Gb downloads

Submitted by: Gene Expression Omnibus (GEO)
Study: Long non-coding RNA Linc-RAM enhances myogenic differentiation by interacting with MyoD
show Abstracthide Abstract
Long non-coding RNAs are important regulators of diverse biological prosesses. Here, we report on functional identification and characterization of a novel long intergenic noncoding RNA with MyoD-regulated and skeletal muscle-restricted expression that promotes the activation of the myogenic program, and is therefore termed Linc-RAM (Linc-RNA Activator of Myogenesis). Linc-RAM is transcribed from an intergenic region of myogenic cells and its expression is upregulated during myogenesis. Notably, in vivo functional studies show that Linc-RAM knockout mice display impaired muscle regeneration due to differentiation defect of satellite cells. Mechanistically, Linc-RAM regulates expression of myogenic genes by directly binding MyoD, which in turn promotes the assembly of the MyoD-Baf60c-Brg1 complex on the regulatory elements of target genes. Collectively, our findings reveal the functional role and molecular mechanism of a lineage-specific Linc-RAM as a regulatory lncRNA required for tissues-specific chromatin remodeling and gene expression. Overall design: We first investigated the global effect of Linc-RAM on gene expression by RNA-Seq analysis during myogenic differentiation using RNAs isolated from differentiating C2C12 myoblasts in which Linc-RAM was either stably overexpressed or knocked down.
Sample: C2C12_control
SAMN04025653 • SRS1051798 • All experiments • All runs
Organism: Mus musculus
Instrument: Illumina HiSeq 2000
Strategy: RNA-Seq
Selection: cDNA
Layout: PAIRED
Construction protocol: RNA was harvested using Trizol reagent RNA libraries were prepared for sequencing using standard Illumina protocols
Experiment attributes:
GEO Accession: GSM1865851
Runs: 1 run, 26.3M spots, 5.3G bases, 3.3Gb
Run# of Spots# of BasesSizePublished


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