Metagenome-seq of deep-sea sediment from Indian Ocean - SRA

SRX18156628: Metagenome-seq of deep-sea sediment from Indian Ocean
1 ILLUMINA (Illumina NovaSeq 6000) run: 54M spots, 15.4G bases, 4.7Gb downloads

Design: A total amount of 1g DNA per sample was used as input material for the DNA sample preparations. Sequencing libraries were generated using NEBNext Ultra DNA Library Prep Kit for Illumina (NEB, USA) following manufacturers recommendations and index codes were added to attribute sequences to each sample. Briefly, the DNA sample was fragmented by sonication to a size of 350 bp, then DNA fragments were end-polished, A-tailed, and ligated with the full-length adaptor for Illumina sequencing with further PCR amplification. At last, PCR products were purified (AMPure XP system) and libraries were analyzed for size distribution by Agilent2100 Bioanalyzer and quantified using real-time PCR. The clustering of the index-coded samples was performed on a cBot Cluster Generation System according to the manufacturers instructions. After cluster generation, sequencings were carried out on Illumina NovaSeq platform in Novogene Corporation (Beijing, P. R. China).

Submitted by: Zhejiang Sci-Tech University

Study: Metagenomic sequencing of marine samples Raw sequence reads

PRJNA776705 • SRP344129 • All experiments • All runs

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This project aims to obtain metagenomic sequences and metagenome-assembled genomes from multiple marine environments.

Sample: TVG05

SAMN31582573 • SRS15656804 • All experiments • All runs

Organism: marine metagenome

Library:

Name: TVG05

Instrument: Illumina NovaSeq 6000

Strategy: WGS