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SRX1101050: GSM1826873: Neuron replicate 2; Drosophila melanogaster; RNA-Seq
1 ILLUMINA (Illumina HiSeq 2500) run: 43.2M spots, 4.1G bases, 2.5Gb downloads

Submitted by: Gene Expression Omnibus (GEO)
Study: Transcriptomes of lineage-specific Drosophila neuroblasts profiled via genetic targeting and robotic sorting
show Abstracthide Abstract
A brain consists of numerous distinct neurons arising from a limited number of progenitors, called neuroblasts in Drosophila. Each neuroblast makes a specific neuronal lineage. To unravel the transcriptional networks that underlie the development of distinct neuroblast lineages, we marked and isolated lineage-specific neuroblasts for RNA sequencing. We labeled particular neuroblasts throughout neurogenesis by activating a conditional neuroblast driver in specific lineages using various intersection strategies. The targeted neuroblasts were efficiently recovered using a custom-built device for robotic single cell picking. Transcriptome analysis on the mushroom body, antennal lobe, and type II neuroblasts besides non-selective neuroblasts, neurons, and glia revealed a rich repertoire of transcription factors expressed among neuroblasts in diverse patterns. In addition to those likely pan-neuroblast transcription factors, there exist many transcription factors selectively enriched or repressed in certain neuroblasts. The unique combinations of transcription factors present in different neuroblasts may govern the diverse lineage-specific neuron fates Overall design: Examination of 5 different cell types
Sample: Neuron replicate 2
SAMN03890829 • SRS995668 • All experiments • All runs
Instrument: Illumina HiSeq 2500
Strategy: RNA-Seq
Selection: cDNA
Layout: SINGLE
Construction protocol: 30 fly larvae brain tissues were dissected at defined ages, digested with a mixture of pronase(1mg/ml) and dispase (1mg/ml) for 30 min at 25C, washed, triturated and 10 GFP labeled cells were sorted with a robotic sorter and stored at -80C in XB buffer from PicoPure kit (life technologies, KIT0204). Total RNA from around 100 pooled cells was extracted using PicoPure kit. Extracted RNA was amplified using Ovation RNA-Seq System V2 (NuGEN, #7102). 1~1.5 ug of the amplified material was used for library construction using Encore NGS Multiplex System (NuGEN, #0314).
Experiment attributes:
GEO Accession: GSM1826873
Runs: 1 run, 43.2M spots, 4.1G bases, 2.5Gb
Run# of Spots# of BasesSizePublished


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