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SRX1074530: GSM1723724: totalRNA22-1; Arabidopsis thaliana; RNA-Seq
1 ILLUMINA (Illumina HiSeq 2000) run: 20.9M spots, 4.2G bases, 2.4Gb downloads

Submitted by: Gene Expression Omnibus (GEO)
Study: Time-course transcriptome of wild-type Arabidopsis leaf
show Abstracthide Abstract
During the senescence stage, leaves undergo degeneration and relocate nutrients accumulated during the growth stage to sink parts such as seeds, critically contributing to plants’ productivity and fitness. Here, we asked how leaf transcriptome is regulated during the senescence stage by performing directional sequencing of total and small RNAs for the entire lifespan of Arabidopsis leaves. Overall design: The total RNA and small RNA profiles of the Arabidopsis leaf along lifespan (14 and 7 time points, respectively) were generated by next-generation sequencing using Illumina Hiseq2000. Two independent biological replicates were used for each experiment.
Sample: totalRNA22-1
SAMN03798053 • SRS973210 • All experiments • All runs
Instrument: Illumina HiSeq 2000
Strategy: RNA-Seq
Selection: cDNA
Layout: PAIRED
Construction protocol: RNA was isolated using WelPrepTM (WelGENE, Korea). Total RNA integrity was checked using an Agilent Technologies 2100 Bioanalyzer with an RNA Integrity Number (RIN) value greater than 8.5 RNA libraries were prepared for sequencing using standard Illumina protocols
Experiment attributes:
GEO Accession: GSM1723724
Runs: 1 run, 20.9M spots, 4.2G bases, 2.4Gb
Run# of Spots# of BasesSizePublished


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