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SRX1068206: GSM1717425: WT 3; Mus musculus; RNA-Seq
1 ILLUMINA (Illumina HiSeq 2000) run: 28.1M spots, 5.6G bases, 3.2Gb downloads

Submitted by: Gene Expression Omnibus (GEO)
Study: Signaling through MyD88 in bone marrow-derived cells promotes gastric tumorigenesis by inducing the TLR2/CD14 pathway in tumor cells
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Gan mice express Wnt1, Ptgs2, and Ptges, which develop inflammation-associated gastric tumors (Oshima et al, Gastroenterology 131: 1086, 2006). We examined the role of MyD88 in tumorigenesis by construction of Myd88-/- Gan mice and bone marrow transplantation into Gan mice from Myd88-/- mice. Overall design: Total RNA was prepared from wild-type normal glandular stomach (n=3: WT 1–WT 3), B6 C2mE mice (n=3: C2mE 1–C2mE 3), B6 Gan mice (n=3: Gan1–Gan3), B6 Gan MyD88-/- mice (n=3: Gan 1 (MyD88-/-)–Gan 3 (MyD88-/-)), and B6 bone marrow transplanted Gan mice from Myd88-/- mice (n=3: BMT-Gan 1 (from MyD88-/-)–BMT-Gan 3 (from MyD88-/-)). We used Illumina HiSeq 2000, and examined expression profiles.
Sample: WT 3
SAMN03785209 • SRS968105 • All experiments • All runs
Organism: Mus musculus
Instrument: Illumina HiSeq 2000
Strategy: RNA-Seq
Selection: cDNA
Layout: PAIRED
Construction protocol: Total RNA was extracted using ISOGEN (Nippon Gene) and purified using Rneasy Mini Kit (Quiagen) RNA libraries were prepared for sequencing using standard Illumina protocols
Experiment attributes:
GEO Accession: GSM1717425
Runs: 1 run, 28.1M spots, 5.6G bases, 3.2Gb
Run# of Spots# of BasesSizePublished


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