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SRX1067044: GSM1716676: Ctl-2; Mus musculus; RNA-Seq
1 ILLUMINA (Illumina HiSeq 2000) run: 72.1M spots, 3.7G bases, 1.4Gb downloads

Submitted by: Gene Expression Omnibus (GEO)
Study: Canonical Wnt signalling regulates nuclear export of Setdb1 during skeletal muscle terminal differentiation [RNA-seq]
show Abstracthide Abstract
The histone 3 lysine 9 methyltransferase Setdb1 is essential for both stem cell pluripotency and terminal differentiation of different cell types. To shed light on Setdb1 roles in these mutually exclusive processes, we used mouse skeletal myoblasts as a model of terminal differentiation. Ex vivo studies on isolated single myofibres showed that Setdb1 is required for muscle adult stem cells expansion following activation. In vitro studies in skeletal myoblasts confirmed that Setdb1 suppresses terminal myoblast differentiation. Genomic binding analyses showed a release of Setdb1 from the promoter of selected target genes upon myoblast terminal differentiation, concomitant to a nuclear export of Setdb1 to the cytoplasm. Both genomic release and cytoplasmic Setdb1 relocalisation during differentiation were dependent on canonical Wnt signalling. Together, our findings revealed Wnt-dependent subcellular relocalisation of Setdb1 as a novel mechanism regulating Setdb1 functions and adult myogenesis. Overall design: RNA-seq of knockdown of Setdb1 in myoblast cells (C2C12).
Sample: Ctl-2
SAMN03783365 • SRS966972 • All experiments • All runs
Organism: Mus musculus
Instrument: Illumina HiSeq 2000
Strategy: RNA-Seq
Selection: cDNA
Layout: SINGLE
Construction protocol: RNA was isolated as described above. Polyadenylated messenger RNA was purified from 1 µg RNA and the library was generated using the TruSeq stranded mRNA library prep Kit, set A and B (Illumina, #RS-122-2101 & #RS-122-2102). The obtained directional libraries were controlled by Bioanalyzer DNA1000 Chips (Agilent, # 5067-1504) and quantified by spectrofluorimetry (QuantiT™ High-Sensitivity DNA Assay Kit, #Q33120, Invitrogen).
Experiment attributes:
GEO Accession: GSM1716676
Runs: 1 run, 72.1M spots, 3.7G bases, 1.4Gb
Run# of Spots# of BasesSizePublished


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