GSM1701340: RNA-seq healthy control before MDP, replicate2; Homo sapi... - SRA

SRX1045337: GSM1701340: RNA-seq healthy control before MDP, replicate2; Homo sapiens; RNA-Seq
1 ILLUMINA (Illumina Genome Analyzer IIx) run: 42.3M spots, 3.2G bases, 1.9Gb downloads

Submitted by: Gene Expression Omnibus (GEO)

Study: Impact of NOD2 Genotype on the Innate Monocyte Transcriptome in Crohn's Disease

PRJNA285507 • SRP058953 • All experiments • All runs

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Short title: Innate Gene/miRNA MDP-response in CD RNA-seq and small RNA-seq were used to identify the expression of gene and microRNA (miRNA) in monocytes from healthy controls and patients with Crohn's Disease (CD), with and without NOD2 (nucleotide-binding oligomerization domain-containing protein 2) single nucleotide polymorphisms (SNPs) after stimulation with muramyl dipeptide (MDP). Overall design: Identification of the gene expression of mRNA and miRNA in monocytes from healthy controls and Crohn's patients with and without NOD2 SNPs before and after stimulation with the NOD2-agonist MDP.

Sample: RNA-seq healthy control before MDP, replicate2

SAMN03754633 • SRS950584 • All experiments • All runs

Organism: Homo sapiens

Library:

Instrument: Illumina Genome Analyzer IIx

Strategy: RNA-Seq

Source: TRANSCRIPTOMIC

Selection: cDNA

Layout: SINGLE

Construction protocol: Total RNA from unstimulated and MDP-stimulated monocytes was prepared using NucleoSpin RNA II isolation kit (Macherey-Nagel). Illumina TruSeq Standard RNA Sample Prep was used with 1.5 ug of total RNA for the construction of sequencing libraries.

Experiment attributes:

GEO Accession: GSM1701340

Links:

External link: GEO Sample GSM1701340

NCBI link: NCBI Entrez (gds)

Runs: 1 run, 42.3M spots, 3.2G bases, 1.9Gb

Run	# of Spots	# of Bases	Size	Published
SRR2047009	42,265,772	3.2G	1.9Gb	2017-02-15

ID:: 1516631

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