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SRX1020497: RNA-Seq data of human adult kidney tissues (2)
1 ILLUMINA (Illumina HiSeq 2000) run: 80.1M spots, 12.2G bases, 7.2Gb downloads

Design: 2 different fetal normal tissues and 6 different adult normal tissues were purchased from different sources (Agilent, Biochain and OriGene). The qualities of these total RNA were tested using the Agilent Bioanalyser 2100 Eukaryote Total RNA Nano Series II. Only total RNAs with a RIN score of more than 7 were used for RNA-Seq library construction. Ribosomal RNA (rRNA) was removed from total RNA using the RiboMinus™ Eukaryote Kit for RNA-Seq from Ambion. The ribosomal RNA depleted RNA fraction is termed the RiboMinus™ RNA fraction and is enriched in polyadenylated (polyA) mRNA, non-polyadenylated RNA, pre-processed RNA, tRNA, and may also contain regulatory RNA molecules such as microRNA (miRNA) and short interfering RNA (siRNA), snRNA, and other RNA transcripts of yet unknown function. Ambion RiboMinus rRNA depletion was performed as described in the manufacturer’s protocol (Pub. Part no.: 100004590, Rev. date 2 December 2011) following the standard protocol. TruSeq RNA Sample Preparation was performed on the RiboMinus™ RNA fraction as described in the manufacturer’s protocol (Pub. Part no.: 15026495 Rev. F March 2014) following the low sample protocol. The libraries were sequenced on Illumina’s HiSeq 2000 instrument following standard protocol.
Submitted by: Cancer Science Institute of Singapore
Study: An atlas of ribosomal RNA-depleted RNA-Seq data from different normal adult and fetal human tissues
show Abstracthide Abstract
Gene expression is the most fundamental level at which the genotype leads to the phenotype of the organism. Enabled by ultra-high-throughput next-generation DNA sequencing, RNA-Seq involves shotgun sequencing of fragmented RNA transcripts by next-generation sequencing followed by in silico assembly, and is rapidly becoming the most popular method for gene expression analysis. Poly[A]+ RNA-Seq analyses of normal human adult tissue samples such as Illumina s Human BodyMap 2.0 Project and the RNA-Seq atlas have provided a useful global resource and framework for comparisons with diseased tissues such as cancer. However, these analyses have failed to provide information on poly[A]- RNA, which is abundant in our cells. The most recent advances in RNA-Seq analyses, use ribosomal RNA-depletion to provide information on both poly[A] and poly[A]- RNA. In this paper, we describe the use of Illumina s HiSeq 2000 to generate high quality rRNA-depleted RNA- Seq sequence datasets from human fetal and adult tissues. The datasets reported here will be useful in demonstrating the different expression profiles in different tissues.
Sample: Biochain_Adult_Kidney
SAMN03610552 • SRS931040 • All experiments • All runs
Organism: Homo sapiens
Library:
Name: Biochain_Adult_Kidney
Instrument: Illumina HiSeq 2000
Strategy: RNA-Seq
Source: TRANSCRIPTOMIC
Selection: other
Layout: PAIRED
Spot descriptor:
forward77  reverse

Runs: 1 run, 80.1M spots, 12.2G bases, 7.2Gb
Run# of Spots# of BasesSizePublished
SRR201424080,053,02212.2G7.2Gb2015-05-25

ID:
1477540

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