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SRX705987: GSM1507953: WT siliques replicate 1; Arabidopsis thaliana; RNA-Seq
1 ILLUMINA (Illumina HiSeq 2000) run: 38.7M spots, 3.9G bases, 2.5Gb downloads

Submitted by: Gene Expression Omnibus (GEO)
Study: Identification of CLF-regulated transcripts using strand-specific RNA-sequencing in Arabidopsis
show Abstracthide Abstract
CURLY LEAF (CLF), the major histone methyltransferase of Polycomb Repressive Complex 2 (PRC2), modifies trimethylation of histone H3 lysine 27 (H3K27me3) and mediates dynamical chromatin repression in Arabidopsis. Here we used strand specific RNA-sequencing to profile Arabidopsis transcriptomes obtained from roots, shoots, flowers and siliques of Col-0 and clf-28 plants. Our analysis identified a large number of CLF-regulatedd transcripts in Arabidopsis. Overall design: Transcriptome profiling in roots, shoots, inflorescences and siliques of WT and clf-28 plants with 3 biological replicates.
Sample: WT siliques replicate 1
SAMN03073869 • SRS705536 • All experiments • All runs
Library:
Instrument: Illumina HiSeq 2000
Strategy: RNA-Seq
Source: TRANSCRIPTOMIC
Selection: cDNA
Layout: SINGLE
Construction protocol: All samples were harvested and frozen immediately in liquid nitrogen until use. Total RNA was extracted and treated with DNase I using RNeasy Plant mini kit (Qiagen). RNA sequence libraries were prepared with TruSeq strand-specific RNA sample Prep kit according to the instructions (Illumina). Sequences were determined by HiSeq 2000 sequencing system in the genomic center in Rockefeller University.
Experiment attributes:
GEO Accession: GSM1507953
Links:
External link:
Runs: 1 run, 38.7M spots, 3.9G bases, 2.5Gb
Run# of Spots# of BasesSizePublished
SRR158056338,741,8583.9G2.5Gb2016-03-28

ID:
994656

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