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SRX12006855: Oxford nanopore (ONT) sequence data from metagenome of Halichondria panicea: pinacoderm and choanosome
1 OXFORD_NANOPORE (MinION) run: 1.3M spots, 3.4G bases, 2.8Gb downloads

Design: Sequencing was performed following the ONT 1D genomic DNA by ligation protocol (SQK-LSK109), using 1 g of DNA with the L fragment buffer to enrich DNA fragments over 3 kb. CleanNGS magnetic beads (CleanNA, Netherlands) were used, but otherwise the ONT protocol (SQK-LSK109) was followed. The library was sequenced on a MinION sequencer using a FLO-MINSP6 flow cell (ONT, UK). After sequencing, base calling for 1D reads was performed using Guppy (v2.3.5, ONT).
Submitted by: University of Southern Denmark
Study: Halichondria panicea draft genome and microbiome metagenome assembled genomes (MAGs)
show Abstracthide Abstract
These data were generated in order to create a reference metagenome for the emerging model sponge species, Halichondria panicea and its microbiome. These data include raw sequences from three separate sequencing runs of the metagenome of a single individual of Halichondria panicea - one Illumina MiSeq (2x300 bp, paired-end, across two lanes) run and three Oxford Nanopore Technologies (ONT) long-read sequencing runs, generating 53.8 and 7.42 Gbp respectively. Draft metagenome assemblies were created using the Illumina, ONT and both approaches combined. Metagenome assembled genomes (MAGs) for the sponge and two microbial symbionts were created from bining the Illumina-only metagenome assembly.
Sample:
SAMN20669593 • SRS10013166 • All experiments • All runs
Library:
Name: H_panicea_ONT_1
Instrument: MinION
Strategy: WGS
Source: METAGENOMIC
Selection: RANDOM
Layout: SINGLE
Runs: 1 run, 1.3M spots, 3.4G bases, 2.8Gb
Run# of Spots# of BasesSizePublished
SRR157111361,262,7213.4G2.8Gb2021-10-12

ID:
15925745

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