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SRX12006857: Oxford nanopore (ONT) sequence data from metagenome of Halichondria panicea: pinacoderm and choanosome
1 OXFORD_NANOPORE (MinION) run: 699,326 spots, 2.3G bases, 2Gb downloads

Design: Whole genome amplification (WGA) using the REPLI-g Mini Kit (Qiagen, Germany) was performed on DNA from H. panicea. This DNA was sequenced along with samples from E. coli (unamplified), E. coli (WGA), and water (WGA) using the ONT SQK-LSK9 barcoding protocol with the EXPNBD104 kit and the barcoded DNA from these four samples DNA was pooled. After sequencing, base calling for 1D reads was performed using Guppy (v2.3.5, ONT). The submitted sequences have been quality-filtered, demultiplexed, and trimmed of adaptor sequences.
Submitted by: University of Southern Denmark
Study: Halichondria panicea draft genome and microbiome metagenome assembled genomes (MAGs)
show Abstracthide Abstract
These data were generated in order to create a reference metagenome for the emerging model sponge species, Halichondria panicea and its microbiome. These data include raw sequences from three separate sequencing runs of the metagenome of a single individual of Halichondria panicea - one Illumina MiSeq (2x300 bp, paired-end, across two lanes) run and three Oxford Nanopore Technologies (ONT) long-read sequencing runs, generating 53.8 and 7.42 Gbp respectively. Draft metagenome assemblies were created using the Illumina, ONT and both approaches combined. Metagenome assembled genomes (MAGs) for the sponge and two microbial symbionts were created from bining the Illumina-only metagenome assembly.
Sample:
SAMN20669593 • SRS10013166 • All experiments • All runs
Library:
Name: H_panicea_ONT_3
Instrument: MinION
Strategy: WGA
Source: METAGENOMIC
Selection: RANDOM
Layout: SINGLE
Runs: 1 run, 699,326 spots, 2.3G bases, 2Gb
Run# of Spots# of BasesSizePublished
SRR15711134699,3262.3G2Gb2021-10-12

ID:
15925747

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