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SRX7613742: GSM4278346: WT IFM 1d rep_2; Drosophila melanogaster; RNA-Seq
1 ILLUMINA (Illumina HiSeq 2000) run: 150.1M spots, 15G bases, 5.2Gb downloads

Submitted by: NCBI (GEO)
Study: Muscle-type specific transcriptomic expression patterns in Drosophila
show Abstracthide Abstract
To complement our existing data on developmental gene expression changes in flight muscle (IFM) development in Drosophila (GSE107247, GSE63707), we performed mRNA-Seq on dissected leg samples at three stages during pupal development (30, 50 and 72h APF). We further sequenced an additional timepoint at 24h APF for RNAi knockdown of aret (Bru1) in flight muscle. Comparison of splicing and expression profiles of sarcomeric genes allowed us to identify muscle-type specific differences in gene and isoform expression between fibrillar flight muscle and tubular leg muscle. We can further trace the dynamics of exon usage in sarcomere genes across the developmental timecourse, allowing us to identify events the switch during muscle differentiation and maturation. Overall design: Design 1: aret (Bru1) RNAi IFM at 24h APF (versus WT IFM at 24h APF from GSE107247); Design 2: timecourse of whole leg gene expression at imaginal disc (myoblast), 30h, 50h and 72h APF.
Sample: WT IFM 1d rep_2
SAMN13898462 • SRS6047279 • All experiments • All runs
Instrument: Illumina HiSeq 2000
Strategy: RNA-Seq
Selection: cDNA
Layout: SINGLE
Construction protocol: Total RNA isolated in Tri-Pure reagent (phenol-chloroform), mRNA selected via 3 passes over Invitrogen oligo-dT magnetic beads, mRNA was fragmented using heat and fragmentation buffer, RT was performed with Invitrogen SSIII first strand synthesis system and second-strand labeled with dUTP. RNA libraries were prepared for sequencing by the IMP sequencing core facility (Vienna, Austria) using standard Illumina protocols.
Experiment attributes:
GEO Accession: GSM4278346
Runs: 1 run, 150.1M spots, 15G bases, 5.2Gb
Run# of Spots# of BasesSizePublished


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