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SRX000179: 454 sequencing of Escherichia coli UTI89 genomic fragment library
6 LS454 (454 GS FLX) runs: 459,708 spots, 118.2M bases, 276.5Mb downloads

Design: none provided
Submitted by: The Genome Center at Washington University School of Medicine in St. Louis (WUGSC)
Study: Uropathogenic strain
show Abstracthide Abstract
<i><B>Escherichia coli</i> strain UTI89</B>. This strain (UTI89) is a uropathogenic strain isolated from a woman with uncomplicated cystitis (bladder inflammation) that has been demonstrated to cause cystitis in a murine urinary tract infection model. The genome of UTI89 showed 4 pathogenicity islands. A plasmid was found that was similar to the F plasmid but which also contained virulence genes. Comparison of a set of orthologs from this organism with other <i>E. coli</i> strains showed positive selection on some genes, including those for iron acquisition. Comparison with additional sequences (found in Accession Numbers DQ389000:DQ389068 and DQ440980:DQ441250) of a subset of genes from a number of isolates from patients with UTI infections and isolates from healthy individuals showed positive selection of <i>fepE</i> and <i>amiA<i> genes and some weakly positive selection for <i>ompC</i>.
Sample: Escherichia coli UTI89 genomic isolate
SAMN00000110 • SRS000341 • All experiments • All runs
Library:
Name: 2121724656
Instrument: 454 GS FLX
Strategy: WGS
Source: GENOMIC
Selection: RANDOM
Layout: SINGLE
Spot descriptor:
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Experiment attributes:
submission_id: SRA000156
Pipeline: show...hide...
Program
454BaseCaller Base Caller
454BaseCaller Quality Scorer
Runs: 6 runs, 459,708 spots, 118.2M bases, 276.5Mb
Run# of Spots# of BasesSizePublished
SRR0008736,6951.7M4Mb2008-04-05
SRR0008721,133292,869747,865b2008-04-05
SRR0008713,164812,7481.9Mb2008-04-05
SRR000870216,45855.6M129.5Mb2008-04-05
SRR0008691,741448,1001.1Mb2008-04-05
SRR000868230,51759.3M139.3Mb2008-04-05

ID:
180

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