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SRX481054: female rectal cancer carcinoma and adjacent normal tissue SAPAS transcriptome
2 ILLUMINA (Illumina Genome Analyzer IIx) runs: 56.7M spots, 3.3G bases, 1.9Gb downloads

Design: Library was constructed as described previously (PMID:21474764). Briefly, total RNA was extracted from carcinoma and adjacent normal tissue of 53 female of stage II rectal cancer patient by TRIzol, and 5-10 mg of total RNA was randomly fragmented by heating. An anchored oligo d(T) primer and a 59 template switching linker tagged with Illumina adaptors were used in template switch reverse transcription (RT) by SuperScript II reverse transcriptase from Invitrogen. Two mutations in the poly(A) were introduced by PCR amplification with a determined number of cycles to ensure that the ds cDNA remain in the exponential phase of amplification. The PCR products were recovered after PAGE size selection for 250–400 bp by PAGE gel-excision. The recovery product was quantified by Agilent 2100 bioanalyzer and submit to Illumina Genome Analyzer II sequencing.
Submitted by: School of Life Sciences, Sun Yat-sen University
Study: Homo sapiens Transcriptome or Gene expression
show Abstracthide Abstract
Rectal cancer stage II carcinoma and adjacent normal tissue transcriptome
Sample: female rectal cancer carcinoma and adjacent normal tissue
SAMN02673190 • SRS565828 • All experiments • All runs
Organism: Homo sapiens
Library:
Name: Rectal R12 carcinoma and adjacent normal tissue SAPAS
Instrument: Illumina Genome Analyzer IIx
Strategy: RNA-Seq
Source: TRANSCRIPTOMIC
Selection: PolyA
Layout: SINGLE
Spot descriptor:
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Runs: 2 runs, 56.7M spots, 3.3G bases, 1.9Gb
Run# of Spots# of BasesSizePublished
SRR118264427,152,7401.6G942.6Mb2015-07-22
SRR118264529,568,3901.7G1Gb2015-07-22

ID:
671127

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