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SRX011561: Seasonally dynamic fungal communities in Quercus macrocarpa phyllosphere differ among urban and rural environments
1 LS454 (454 GS FLX) run: 105,838 spots, 27.9M bases, 53.4Mb downloads

Design: Fungus-specific ITS1F and ITS4 primers were used to amplify the ITS region flanked by the 18S and 25S ribosomal RNA genes. the amplicons were directly 454-sequenced in reverse direction using a primer that incorporated the ITS4 and the 454-sequencing A primer.
Submitted by: Kansas State University - Fungal Ecology Lab (KSU-FEL)
Study: Targeted gene survey from fungal communities inhabiting Quercus macrocarpa phyllosphere
show Abstracthide Abstract
This project estimates fungal species richness and diversity in temperate deciduous trees and tests hypotheses on urbanization's effects on the richness and structure of the leaf associated communities. This study targeted the fungal communities inhabiting the phyllosphere of Quercus macrocarpa in urban and non-urban environments within and outside of Manhattan, Kansas. The study combined massively parallel 454 sequencing of the Internal Transcribed Spacer 1 (ITS1) amplicons with DNA tagging to compare fungal species richness, diversity and the community composition among conspecific trees located in urban and adjacent rural environments. The study uses an experimental design with the land use as main effect and the sites nested within the land use. The data clearly show that fungal phyllosphere communities are diverse and that richness and diversity were significantly and substantially lower in the urban environment. <a href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?holding=&db=Nucleotide&cmd=search&term=FJ756950:FJ763141[accn]" >FJ756950-FJ763141 </a> are ribosomal RNA sequences associated with this project.
Sample: Generic sample from Fungi
SAMN00003850 • SRS006137 • All experiments • All runs
Name: Pool#4.FJSKC4L04.sff
Instrument: 454 GS FLX
Strategy: AMPLICON
Source: OTHER
Selection: RANDOM
Layout: SINGLE
Spot descriptor:

Runs: 1 run, 105,838 spots, 27.9M bases, 53.4Mb
Run# of Spots# of BasesSizePublished


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