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Design: Chickspress
Submitted by: UNIVERSITY OF ARIZONA
Study: Chickspress
show Abstracthide Abstract
Lack of comprehensive genome structural- and functional-annotation significantly prevents researchers from exploiting the full potential of the chicken genome model system for comparative and functional genomic studies. Comprehensive genome structural- and functional-annotation must be made easily accessible for the chicken genome sequence to reach its full potential to contribute to human health and well-being. To this end we collected a variety of tissues from 2 (1 male, 1 female) healthy 2-year-old red jungle fowls. We performed mRNA sequencing, small RNA sequencing, proteomics and proteogeomic mapping on each of these tissues to further elucidate tissue and sex specific expression and to improve upon current gene models. Results of small RNA sequencing have also been deposited at ArrayExpress under accession number E-MTAB-1826 ( https://www.ebi.ac.uk/arrayexpress/experiments/E-MTAB-1826/ ).
Library:
Name: heart_female_mRNA
Instrument: Illumina HiSeq 2000
Strategy: RNA-Seq
Source: TRANSCRIPTOMIC
Selection: cDNA
Layout: PAIRED
Construction protocol: The source of the RNA was a variety of red jungle fowl tissues. Total RNA was extracted according to the Qiagen miRNAeasy protocol. MRNAseq libraries were constructed according to the Illumina TruSeq RNA protocol.
Spot descriptor:
forward101  reverse

Experiment attributes:
Experimental Factor: sex: female
Experimental Factor: organism part: heart
Run# of Spots# of BasesSizePublished
ERR348569unavailable2013-10-16

ID:
515486

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