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Design: Sanger_zebrafish_sequencing
Submitted by: The Wellcome Trust Sanger Institute (SC)
Study: Sanger_zebrafish_sequencing
Name: ZFswimbladder_2_RNA 1523495
Instrument: Illumina Genome Analyzer II
Strategy: RNA-Seq
Selection: cDNA
Layout: PAIRED
Construction protocol: Zebrafish tissue was collected from Singapore strain incross fish grown at 28C. Collected samples were snap frozen on dry ice and stored at -70 C Total RNA was extracted using Trizol Reagent (Invitrogen) following the manufacturer's instructions. Pellets were re-suspended in 10 mM Tris pH 7.5 and the RNA was quantified using a NanoDrop ND-1000 Spectrophotometer (Axon Instruments). Total RNA was enriched for polyA+ RNA by 2 rounds of polyA pull down with magnetic beads and included a DNase treatment between the 2 rounds. RNA was chemically fragmented, LiCl precipitated, reverse transcribed with random primers, a second strand synthesized and made into a standard Illumina library with a fragment size of 250 to 300 bp.
Spot descriptor:
forward         85  reverse

Experiment attributes:
Experimental Factor: ORGANISM_PART: swim bladder
Run# of Spots# of BasesSizePublished


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