show Abstracthide AbstractGeomyces pannorum is a haploid, asexual fungus which can tolerate low temperature and high salinity conditions. We extracted 14 individuals from permafrost and from temperate environments and obtained de novo draft assembly for each of them. Comparative genome analysis reveals extremely high nucleotide diversity (~0.5 synonymous substitutions between individuals) and also reveals certain regions with phylogenetic pattern different from the rest of the genome.