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SRX13850419: Metagenome of Limnoraphis bloom in Willow Creek Reservoir
1 PACBIO_SMRT (Sequel II) run: 97,062 spots, 812.4M bases, 413.4Mb downloads

Design: Material collected by filtration onto 1.2 um filter was subjected to DNA extraction using a Qiagen DNeasy PowerBiofilm kit. DNA (1 ug) at peak size of 8.4 kbp was used without shearing for PacBio Sequel II SMRTbell template preparation with barcode adapter triplex sequencing using standard PacBio protocols. Q20 CCS reads were extracted.
Submitted by: Oregon State University
Study: Genomes of bloom-forming freshwater cyanobacteria Dolichospermum sp., Aphanizomenon flos-aquae, Gloeotrichia sp.and bloom-associated heterotrophic bacteria
show Abstracthide Abstract
Cyanobacterial blooms are an increasing scourge in freshwater lakes and reservoirs across the world. They can be toxic and represent ecological disfunction. This project determines the genome sequences of the standing populations of such bloom-forming cyanobacteria, mainly from the Pacific Northwest region of the US. The sequences obtained allow the deduction of gene content (such as toxin biosynthetic genes), relationships across geographic areas, and population structures within blooms. They also provide taxonomic clarification among bloom-forming cyanobacteria. Genomes of bacteria associated with these blooms are also being sequenced. These bacteria may have synergistic, commensal or predatory relationships with cyanobacterial blooms. An understanding of these relationships will provide insight into the development and turnover of cyanobacterial blooms.
Sample: Limnoraphis_WC205
SAMN18685712 • SRS11727290 • All experiments • All runs
Library:
Name: WC205
Instrument: Sequel II
Strategy: WGS
Source: METAGENOMIC
Selection: RANDOM
Layout: SINGLE
Runs: 1 run, 97,062 spots, 812.4M bases, 413.4Mb
Run# of Spots# of BasesSizePublished
SRR1768715997,062812.4M413.4Mb2022-01-21

ID:
19302392

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