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SRX8149426: tufA DNA metabarcoding of U. fasciata: tissue
1 OXFORD_NANOPORE (MinION) run: 11,472 spots, 9.4M bases, 8.3Mb downloads

Design: Ligation with multiplexing
Submitted by: Ariel University
Study: MinION DNA barcoding of five representative species
show Abstracthide Abstract
To test the accuracy and the efficiency of DNA barcoding with MinION nanopore we amplified the relevant barcode regions from the genomic DNA of five representative species; Escherichia coli (16S rRNA gene), Homo sapiens (COI), Gracilaria cearensis (18S rRNA gene), Ulva fasciata (tufA) and Saccharomyces cerevisiae (ITS), representing bacteria, metazoans, green and red algae as well as fungi, respectively. The amplicons were sequenced with MinION using ligation protocol with multiplexing that was followed by a bioinformatics pipeline that was adapted from Maestri et al. (2019)
Sample:
SAMN14651238 • SRS6512061 • All experiments • All runs
Organism: Ulva fasciata
Library:
Name: tufA
Instrument: MinION
Strategy: AMPLICON
Source: GENOMIC
Selection: PCR
Layout: SINGLE
Runs: 1 run, 11,472 spots, 9.4M bases, 8.3Mb
Run# of Spots# of BasesSizePublished
SRR1158174011,4729.4M8.3Mb2020-04-21

ID:
10626659

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