GSM4569909: FOXA1_UW_PDX_86-2; Homo sapiens; ChIP-Seq - SRA

SRX8406530: GSM4569909: FOXA1_UW_PDX_86-2; Homo sapiens; ChIP-Seq
1 ILLUMINA (NextSeq 500) run: 20M spots, 1.5G bases, 560.9Mb downloads

Submitted by: NCBI (GEO)

Study: Charting the prostate epigenomic landscape from transformation to progression

PRJNA540151 • SRP194063 • All experiments • All runs

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We generate transcription factor, histone modification and ATAC cistromes in nomal prostate epithelium, primary prostate tumor and metastatic prostate cancer human specimens Overall design: ChIP-seq for AR, FOXA1, HOXB13, H3K27Ac, H3K4me2, H3K4me3, H3K27me3 and ATAC-seq

Sample: FOXA1_UW_PDX_86-2

SAMN15032464 • SRS6718899 • All experiments • All runs

Organism: Homo sapiens

Library:

Instrument: NextSeq 500

Strategy: ChIP-Seq

Source: GENOMIC

Selection: ChIP

Layout: SINGLE

Construction protocol: One (histone ChIP) or three (transcription factor ChIP) 2 mm2 frozen cores were pulverized using the Covaris CryoPrep system. Tissue was fixed using 1% formaldehyde and chromatin sheared to 300-500 bp in size using the Covaris E220 ultrasonicator. Resulting chromatin was incubated overnight with indicated antibodies. Purified immunoprecipitates were isolated and quantified by Qubit fluorometer. DNA sequencing libraries were prepared using the ThruPLEX-FD Prep kit (Rubicon Genomics). Libraries were sequenced using 75-bp reads on the Illumina platform at the Dana-Farber Cancer Institute.

Experiment attributes:

GEO Accession: GSM4569909

Links:

NCBI link: NCBI Entrez (gds)

Runs: 1 run, 20M spots, 1.5G bases, 560.9Mb

Run	# of Spots	# of Bases	Size	Published
SRR11856278	20,000,000	1.5G	560.9Mb	2020-06-16

ID:: 10937582

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