Instrument: Illumina HiSeq 2000
Strategy: ChIP-Seq
Source: GENOMIC
Selection: ChIP
Layout: SINGLE
Construction protocol: Cells were fixed in 1% formaldehyde for 10 min. Chromatin was prepared and immunoprecipitated as previously described (Greer CB, Tanaka Y, Kim YJ, Xie P, Zhang MQ, Park IH, Kim TH: Histone Deacetylases Positively Regulate Transcription through the Elongation Machinery. Cell Rep 2015, 13(7):1444-1455.). DNA libraries were prepared using ThruPLEX DNA-seq Kit (Takara)