GSM3537883: H9_NPC_input_1; Homo sapiens; ChIP-Seq - SRA

SRX5202459: GSM3537883: H9_NPC_input_1; Homo sapiens; ChIP-Seq
1 ILLUMINA (NextSeq 500) run: 24.5M spots, 1.8G bases, 667.5Mb downloads

Submitted by: NCBI (GEO)

Study: Abundance and localization of human UBE3A isoforms

PRJNA512845 • SRP175161 • All experiments • All runs

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We performed ChIPseq for UBE3A to determine whether it had any direct binding to chromatin in human cells Overall design: ChIPseq for UBE3A and H3K27ac was performed in H9 hESC-derived neuronal progenitors, H9 hESC-derived neurons, and UBE3A-null H9 hESCs using two antibodies against UBE3A.

Sample: H9_NPC_input_1

SAMN10689339 • SRS4207611 • All experiments • All runs

Organism: Homo sapiens

Library:

Instrument: NextSeq 500

Strategy: ChIP-Seq

Source: GENOMIC

Selection: ChIP

Layout: SINGLE

Construction protocol: hESCs, NPCs, and neurons were processed for ChIP-seq as previously described (Cotney & Noonan (2015), Cold Spring Harb Protoc 2015(4): 419 Libraries were prepared from ChIPed DNA using the Takara ThruPLEX DNA-seq library preparation kit according to manufacturer's instructions. Libraries were quantified with the NEBNext Library Quant Kit for Illumina

Experiment attributes:

GEO Accession: GSM3537883

Links:

NCBI link: NCBI Entrez (gds)

Runs: 1 run, 24.5M spots, 1.8G bases, 667.5Mb

Run	# of Spots	# of Bases	Size	Published
SRR8392748	24,522,095	1.8G	667.5Mb	2019-07-02

ID:: 7038158

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