GSM3505820: Oct1 replicate 3; Homo sapiens; ChIP-Seq - SRA

SRX5123056: GSM3505820: Oct1 replicate 3; Homo sapiens; ChIP-Seq
1 ILLUMINA (Illumina NovaSeq 6000) run: 94.2M spots, 28.4G bases, 8.6Gb downloads

Submitted by: NCBI (GEO)

Study: Oct1/Pou2f1 is selectively required for gut regeneration and regulates gut malignancy

PRJNA509072 • SRP173207 • All experiments • All runs

show Abstracthide Abstract

As part of a larger study focused on deletion of Oct1 (Pou2f1) in the mouse gut, we identify Oct1 target genes in HCT116 colon carcinomal cells, with H3K4me3 and input controls. Identified target genes are associated with mitotic stability, metabolism, stress response and malignancy. Overall design: Oct1- or H3K4me3-enriched DNA fragments, and input controls, were sequenced in triplicate

Sample: Oct1 replicate 3

SAMN10536603 • SRS4136371 • All experiments • All runs

Organism: Homo sapiens

Library:

Instrument: Illumina NovaSeq 6000

Strategy: ChIP-Seq

Source: GENOMIC

Selection: ChIP

Layout: PAIRED

Construction protocol: Lysates were clarified from sonicated nuclei and protein-DNA complexes were isolated with antibody (Oct1: Bethyl A310-610A, H3K4me3: Cell Signaling #9727). Qiagen Qiaquick PCR cleanup kit (Cat # 28104). Elution in nuclease-free water. NEBNext ChIP-Seq Library Prep Master Mix Set (Cat # E6240S) with Unique Dual Index Adapters

Experiment attributes:

GEO Accession: GSM3505820

Links:

NCBI link: NCBI Entrez (gds)

Runs: 1 run, 94.2M spots, 28.4G bases, 8.6Gb

Run	# of Spots	# of Bases	Size	Published
SRR8309081	94,174,190	28.4G	8.6Gb	2019-04-19

ID:: 6937043

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Sequence Read Archive

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