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SRX480227: SAPAS library of mouse thymus at 6 post-natal day
1 ILLUMINA (Illumina Genome Analyzer IIx) run: 15.6M spots, 904.2M bases, 460.9Mb downloads

Design: Library was constructed as described previously (PMID:21474764). Briefly, total RNA was extracted from mouse thymus at several different development stages by TRIzol, and 5-10 mg of total RNA was randomly fragmented by heating. An anchored oligo d(T) primer and a 59 template switching linker tagged with Illumina adaptors were used in template switch reverse transcription (RT) by SuperScript II reverse transcriptase from Invitrogen. Two mutations in the poly(A) were introduced by PCR amplification with a determined number of cycles to ensure that the ds cDNA remain in the exponential phase of amplification. The PCR products were recovered after PAGE size selection for 250–400 bp by PAGE gel-excision. The recovery product was quantified by Agilent 2100 bioanalyzer and submit to Illumina Genome Analyzer II sequencing.
Submitted by: School of Life Sciences, Sun Yat-sen University
Study: Mus musculus Thymus Transcriptome from embryo to adult
show Abstracthide Abstract
Total RNA of thymus from mouse different development stages were isolated and deeply sequenced by SAPAS to investigated the global gene regualtion events during thymus genesis and maturation.
Sample: P6
SAMN02670772 • SRS565223 • All experiments • All runs
Organism: Mus musculus
Library:
Name: P6ThymusSAPAS
Instrument: Illumina Genome Analyzer IIx
Strategy: RNA-Seq
Source: TRANSCRIPTOMIC
Selection: PolyA
Layout: SINGLE
Spot descriptor:
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Runs: 1 run, 15.6M spots, 904.2M bases, 460.9Mb
Run# of Spots# of BasesSizePublished
SRR118145315,589,740904.2M460.9Mb2015-07-22

ID:
669661

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