GSM1326797: At_MeDIP_rep1; Arabidopsis thaliana; MeDIP-Seq - SRA

SRX470012: GSM1326797: At_MeDIP_rep1; Arabidopsis thaliana; MeDIP-Seq
1 ILLUMINA (Illumina Genome Analyzer IIx) run: 18.9M spots, 3.7G bases, 2.4Gb downloads

Submitted by: Gene Expression Omnibus (GEO)

Study: Genome-wide profiling of H3K27me1 and 5mC enrichment in young leaves of Arabidopsis thaliana

PRJNA238067 • SRP037728 • All experiments • All runs

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Chromatin, DNA and RNA were extracted from young A. thaliana primary stem leaves. Chromatin and DNA methylation immunoprecipitation experiments were performed using commercially available antibodies and analyzed by Illumina sequencing (ChIP-seq and MeDIP-seq). Overall design: ChIP-seq analysis of H3K27me1 enrichment profiles and MeDIP-seq analysis of 5mC enrichment profiles in 2 biological replicates.

Sample: At_MeDIP_rep1

SAMN02640684 • SRS557244 • All experiments • All runs

Organism: Arabidopsis thaliana

Library:

Instrument: Illumina Genome Analyzer IIx

Strategy: MeDIP-Seq

Source: GENOMIC

Selection: 5-methylcytidine antibody

Layout: PAIRED

Construction protocol: Chromatin and DNA immunoprecipitation was performed essentially as described in Gendrel et al., 2005 and Cortijo et al., 2014, respectively. Total RNA was extracted using RNeasy Mini Kit (Qiagen) according to manufacturers instructions and its quality was validated using RNA 6000 NanoChip (Agilent). 20 ng of immunoprecipitated (IP) and genomic (INPUT) DNA was used to prepare libraries with the ChIP-Seq DNA Sample Prep Kit IP-102-1001. Quality of libraries was validated using 2100 Bioanalyzer (Agilent). Libraries were sequenced with Illumina Genome Analyzer IIx according to manufacturers instructions.

Experiment attributes:

GEO Accession: GSM1326797

Links:

External link: GEO Sample GSM1326797

NCBI link: NCBI Entrez (gds)

Runs: 1 run, 18.9M spots, 3.7G bases, 2.4Gb

Run	# of Spots	# of Bases	Size	Published
SRR1168325	18,876,006	3.7G	2.4Gb	2015-02-04

ID:: 650261

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Sequence Read Archive

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