GSM3172725: David_TC71_SMC1_1; Homo sapiens; ChIP-Seq - SRA

SRX4155879: GSM3172725: David_TC71_SMC1_1; Homo sapiens; ChIP-Seq
1 ILLUMINA (NextSeq 500) run: 32M spots, 2.4G bases, 852Mb downloads

Submitted by: NCBI (GEO)

Study: Genome wide chromatin changes in TC-32 and TC-71 Ewing sarcoma cell lines.

PRJNA474339 • SRP149618 • All experiments • All runs

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We performed ChIPseq on histone modification marks, transcriptional factors and chromatin architectural proteins in TC-32 and TC-71 Ewing sarcoma cell lines. Overall design: ChIPseq profiles of TC-32 and TC-71 Ewing sarcoma cell lines for 3 different marks (BORIS, H3K27ac, SMC1) were generated by next generation sequencing, in duplicate, using Illumina NextSeq 500, for a total of 16 individual samples.

Sample: David_TC71_SMC1_1

SAMN09298502 • SRS3368947 • All experiments • All runs

Organism: Homo sapiens

Library:

Instrument: NextSeq 500

Strategy: ChIP-Seq

Source: GENOMIC

Selection: ChIP

Layout: SINGLE

Construction protocol: Cells were crosslinked with formaldehyde and lysed to obtain purified nuclei that were sonicated to shear chromatin. Protein-DNA complexes were then isolated using Dynabeads with corresponding antibodies, and DNA was isolated through reverse crosslinking and purified by phenol chloroform extraction in order to be used for library construction. Libraries were prepared using the NEBNext Ultra II DNA Library Prep Kit from NEB (E7645), according to the manufacturer's protocol.

Experiment attributes:

GEO Accession: GSM3172725

Links:

NCBI link: NCBI Entrez (gds)

Runs: 1 run, 32M spots, 2.4G bases, 852Mb

Run	# of Spots	# of Bases	Size	Published
SRR7251153	32,049,126	2.4G	852Mb	2019-08-07

ID:: 5642539

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