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SRX4074084: Single cell RNA-sequencing of male C57BL/6j mouse inguinal white adipose tissue lineage marker negative stromal/vascular cells, control.
1 ILLUMINA (NextSeq 500) run: 125.9M spots, 11.6G bases, 4.9Gb downloads

Design: Digest mouse adipose tissue. Collect stromal/vascular cells. Separate into immune and non-immune cells via magnetic cell sorting. Create single cell libraries using the 10X Genomics Chromium Single Cell 3' Library Kit.
Submitted by: Wayne State University School of Medicine
Study: Single-cell RNA-sequencing of white adipose tissue stromal cells during CL-induced adipogenesis
show Abstracthide Abstract
The global incidence of Type 2 diabetes (T2D) has reached epidemic proportions. Although the link between T2D and obesity is undeniable, it is not fully understood how elevated adipose tissue mass leads tot he multiple functional defects that precede overt diabetes. An important gap in our understanding of how obesity promotes metabolic disease is a clear understanding of the cell types that comprise adipose tissue. Recently, technologies have been developed that allow single-cell mRNA-sequencing (scRNA-seq) of thousands of cells, and thereby provide a means of identifying cell types that is largely unbiased and far more comprehensive. Importantly, when combined with previous cell sorting and histochemical analyses, scRNA-seq can identify critical cell subpopulations and states, and infer differentiation trajectories.
Sample: Lineage marker negative stromal/vascular cells from the inguinal white adipose tissue of male C57BL/6j mice.
SAMN09098577 • SRS3291242 • All experiments • All runs
Organism: Mus musculus
Name: iWAT_sham_3D_Linneg
Instrument: NextSeq 500
Strategy: RNA-Seq
Selection: Oligo-dT
Layout: PAIRED
Runs: 1 run, 125.9M spots, 11.6G bases, 4.9Gb
Run# of Spots# of BasesSizePublished


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