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SRX3632920: GSM2973476: Dmel_male_msl2het_H4K16ac_ChIP; Drosophila melanogaster; ChIP-Seq
1 ILLUMINA (Illumina HiSeq 3000) run: 17M spots, 2.5G bases, 871.7Mb downloads

Submitted by: NCBI (GEO)
Study: Facultative dosage compensation of developmental genes on autosomes in Drosophila and mammals [ChIP-seq Dmel]
show Abstracthide Abstract
Haploinsufficiency and aneuploidy are two phenomena, where alteration of gene dosage causes severe cellular defects ultimately resulting in developmental failures and disease. One remarkable exception is the X chromosome, where copy number differences between males and females are buffered through the action of dosage compensation systems. In Drosophila, the Male-Specific Lethal complex (MSLc) mediates two-fold upregulation of the single male X chromosome via Histone H4 lysine 16 acetylation (H4K16ac). The evolutionary origin and conservation of this process orchestrated by MSL2, the only male-specific protein within the fly MSLc, have remained unclear. Here, we report that MSL2, in addition to its function on the X, targets dosage-sensitive autosomal genes involved in patterning and morphogenesis. We show that the precise regulation of these genes by MSL2 is required for proper development of the fly wing. This set of dosage sensitive genes maintained such regulation during evolution, as MSL2 binds and similarly regulates mouse orthologues via deposition of H4K16ac. We propose that MSL2-mediated H4K16ac is an evolutionarily conserved process mediating gene-by-gene dosage compensation across flies and mammals. Overall design: ChIP-seq profiles of various histone modfications and DNA binding proteins in Drosophila melanogaster.. Males and females separated, third instar larvae.
Sample: Dmel_male_msl2het_H4K16ac_ChIP
SAMN08442041 • SRS2899649 • All experiments • All runs
Instrument: Illumina HiSeq 3000
Strategy: ChIP-Seq
Selection: ChIP
Layout: PAIRED
Construction protocol: Larvae were dissected and the front part fixed at RT for 15 min with 1% or 0.2% Formaldehyde. Chromatin was extracted using a sucrose cushion followed by Micrococcal Nuclease treatment and 10 cycles of sonication in a Bioruptor Pico. The lysate was clarified by centrifugation before immunoprecipitation, washes, elution by reverse crosslinking at 65deg for 14h. After RNaseA and ProteinaseK treatment, the DNA from Input and Immunoprecipitation was purified using Phenol-Chloroform Extraction and Ethanol precipitation. NEB Next Ultra II
Experiment attributes:
GEO Accession: GSM2973476
Runs: 1 run, 17M spots, 2.5G bases, 871.7Mb
Run# of Spots# of BasesSizePublished


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