Instrument: Illumina HiSeq 2000
Strategy: ChIP-Seq
Source: GENOMIC
Selection: ChIP
Layout: SINGLE
Construction protocol: Libraries from ChIP samples were made with two different kits. With Illumina (San Diego, CA) TruSeq™ DNA Sample Preparation this modifications were used: ligation with of 1/10th the manufacturers recommended adapter amounts and agarose gel selection of DNA fragments in the 200-500 bp size range. For ThruPLEX (Rubicon Genomics, Ann Arbor, MI, USA. Supplied by Diagenode) library preparatiopn the manufacturer’s instructions were followed. ThruPLEX, Rubicon Genomics