U.S. flag

An official website of the United States government

Format

Send to:

Choose Destination

SRX256891: U2OS GRalpha Input DNA
1 ILLUMINA (Illumina HiSeq 2000) run: 79.9M spots, 16G bases, 10Gb downloads

Design: Input DNA from U2OS cells expressing GRalpha was ligated to indexed TruSeq adapters using index 5.
Submitted by: UNIVERSITY OF CALIFORNIA, SAN FRANCISCO (UCSF)
Study: ChIP-seq of GR in U2OS cells expressing different GR alleles
show Abstracthide Abstract
Glucocorticoid receptor (GR) is a hormone-activated, DNA-binding transcriptional regulatory factor, which regulates diverse aspects of physiology. GR recognizes specifically imperfect palindromic six base pair “half sites" separated by thoursee base pair “spacers”, binding as an inverted dimer. In vivo, different GR target genes depend on different functional surfaces of GR for proper regulation, but rules dictating the relationship between sequence and utilization of distinct GR functional surfaces remain unknown. In this study, we measured changes in gene expression and genomic occupancy upon glucocorticoid treatment of isogenic cultured human cell lines expressing alleles of GR bearing differences in particular functional surfaces.
Sample: Homo sapiens sample GRalpha_input_90m_dex_BS
SAMN01993551 • SRS405483 • All experiments • All runs
Organism: Homo sapiens
Library:
Name: GRalpha_input_90m_dex_BS
Instrument: Illumina HiSeq 2000
Strategy: ChIP-Seq
Source: GENOMIC
Selection: ChIP
Layout: PAIRED
Spot descriptor:
forward101  reverse

Links:
Runs: 1 run, 79.9M spots, 16G bases, 10Gb
Run# of Spots# of BasesSizePublished
SRR79991379,857,12816G10Gb2014-03-25

ID:
358957

Supplemental Content

Search details

See more...

Recent activity

Your browsing activity is empty.

Activity recording is turned off.

Turn recording back on

See more...
Support Center