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SRX256867: U2OS GRalpha ChIP-seq
1 ILLUMINA (Illumina Genome Analyzer II) run: 16.1M spots, 2.4G bases, 1.4Gb downloads

Design: ChIP-seq of U2OS cells expressing GRalpha treated with dexamethasone for 90 minutes. In-house barcodes were used, which were added the 3’ ends of the sequencing adapters and determined using the first four bases of the read. Barcodes are TCAT, GACG, AGTC, and CTGA. The ChIP for GRalpha was split and ligated to each barcode, pooled, and sequenced in one lane.
Submitted by: UNIVERSITY OF CALIFORNIA, SAN FRANCISCO (UCSF)
Study: ChIP-seq of GR in U2OS cells expressing different GR alleles
show Abstracthide Abstract
Glucocorticoid receptor (GR) is a hormone-activated, DNA-binding transcriptional regulatory factor, which regulates diverse aspects of physiology. GR recognizes specifically imperfect palindromic six base pair “half sites" separated by thoursee base pair “spacers”, binding as an inverted dimer. In vivo, different GR target genes depend on different functional surfaces of GR for proper regulation, but rules dictating the relationship between sequence and utilization of distinct GR functional surfaces remain unknown. In this study, we measured changes in gene expression and genomic occupancy upon glucocorticoid treatment of isogenic cultured human cell lines expressing alleles of GR bearing differences in particular functional surfaces.
Sample: Homo sapiens sample GRalpha_ChIP_90m_dex_BS
SAMN01993547 • SRS405466 • All experiments • All runs
Organism: Homo sapiens
Library:
Name: GRalpha_ChIP_90m_dex_BS
Instrument: Illumina Genome Analyzer II
Strategy: ChIP-Seq
Source: GENOMIC
Selection: ChIP
Layout: PAIRED
Spot descriptor:
forward76  reverse

Pipeline: show...hide...
ProgramVersion
seriesoftubes0.9.6.3
Links:
Runs: 1 run, 16.1M spots, 2.4G bases, 1.4Gb
Run# of Spots# of BasesSizePublished
SRR79958816,144,0132.4G1.4Gb2015-07-22

ID:
358933

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