WGS of Arachis hypogaea:Harari - SRA

SRX2105344: WGS of Arachis hypogaea:Harari
1 ILLUMINA (Illumina HiSeq 2500) run: 99.3M spots, 30G bases, 11.4Gb downloads

Design: A group of 20 cultivated genotypes was selected for whole genome re-sequencing including 10 parents of RIL populations (Holbrook et al. 2013) and 10 genotypes with different traits of interest for breeding purposes. The sequencing libraries were constructed using the Illumina TruSeq PCR-free kit starting with 2 µg of total DNA isolated from single plants using Qiagen DNAeasy Plant mini kit® and sheared using Covaris® to obtain 550 bp insert size. The libraries were quantified using Agilent DNA 7500 kit® on the Agilent 2100 Bioanalyzer. Paired-end 150 sequences were generated on an Illumina HiSeq 2500 V4® using 8 lanes for a complete flow cell. The 20 samples were pooled in groups of 10 and each pool was sequenced in 4 lanes for the yield to be more homogeneous. The sequencing data from Tifrunner was obtained from the Peanut Genome Consortium providing ~ 8.4x coverage from 500 bp insert size similarly to the coverage obtained for the samples in the sequencing with average of 10x.

Submitted by: University of Georgia

Study: Arachis hypogaea Raw sequence reads

PRJNA340877 • SRP084297 • All experiments • All runs

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A group of 20 cultivated genotypes was selected for whole genome re-sequencing, including 10 parents of RIL populations (Holbrook et al., 2013) and 10 genotypes with different traits of interest for breeding purposes, to develop a large-scale SNP genotyping array.

Sample: Incomplete pod filling under drought. Extralarge pods

SAMN05721198 • SRS1665960 • All experiments • All runs

Organism: Arachis hypogaea subsp. hypogaea

Library:

Name: Harari

Instrument: Illumina HiSeq 2500

Strategy: WGS

Source: GENOMIC

Selection: RANDOM

Layout: PAIRED

Runs: 1 run, 99.3M spots, 30G bases, 11.4Gb

Run	# of Spots	# of Bases	Size	Published
SRR4124073	99,306,124	30G	11.4Gb	2016-09-07

ID:: 3100124

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Sequence Read Archive

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