GSM2281652: ATL6_H3K27Ac_ChIPSeq; Homo sapiens; ChIP-Seq - SRA

SRX2026374: GSM2281652: ATL6_H3K27Ac_ChIPSeq; Homo sapiens; ChIP-Seq
1 ILLUMINA (Illumina HiSeq 4000) run: 46.7M spots, 4.6G bases, 1.7Gb downloads

Submitted by: NCBI (GEO)

Study: H3K27Ac ChIP-seq for TL-om1 cells and primary ATL cell samples

PRJNA339128 • SRP082212 • All experiments • All runs

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ChIP-seq analysis was performed in adult T-cell leukemia/lymphoma cells to analyze acetylation of histone H3K27. Overall design: TL-Om1 and primary ATL cells were crosslinked with formaldehyde for 10 min. DNA was enriched by chromatin-immunoprecipitation (ChIP) and analyzed by Illumina Hiseq 4000. A sample of whole cell extract (WCE) was sequenced and used as the background to determine enrichment. ChIP was performed using an antibody against histone H3 lysine 27 acetylation (H3K27Ac: Abcam, ab4729).

Sample: ATL6_H3K27Ac_ChIPSeq

SAMN05579856 • SRS1620775 • All experiments • All runs

Organism: Homo sapiens

Library:

Instrument: Illumina HiSeq 4000

Strategy: ChIP-Seq

Source: GENOMIC

Selection: ChIP

Layout: PAIRED

Construction protocol: TL-Om1 cells and primary ATL cells were cross-linked with formaldehyde for 10 min. Lysates were clarified from sonicated nuclei and histone H3K27Ac-DNA complexes were isolated with antibody. A sample of whole cell extract (WCE) was sequenced and used as the background to determine enrichment. Library construction and sequencing by Illumina Hiseq 4000 were performed by BGI Tech Solutions Co., Limited (Hongkong).

Experiment attributes:

GEO Accession: GSM2281652

Links:

NCBI link: NCBI Entrez (gds)

Runs: 1 run, 46.7M spots, 4.6G bases, 1.7Gb

Run	# of Spots	# of Bases	Size	Published
SRR4035098	46,707,184	4.6G	1.7Gb	2017-10-11

ID:: 2925584

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