GSM2267690: ChIP-seq analysis of N-Myc in human HSPCs, untreated, rep... - SRA

SRX2013090: GSM2267690: ChIP-seq analysis of N-Myc in human HSPCs, untreated, replicate 1; Homo sapiens; ChIP-Seq
1 ILLUMINA (Illumina HiSeq 2000) run: 8.9M spots, 654.3M bases, 258.2Mb downloads

Submitted by: NCBI (GEO)

Study: Regulation of stem cell maintenance and lymphoid specification by the ubiquitin ligase Huwe1

PRJNA338631 • SRP081281 • All experiments • All runs

show Abstracthide Abstract

The ubiquitin ligase Huwe1 regulates stem cell quiescence, maintenance and lymphoid specification by controlling the expression of N-Myc. Overall design: Thirty million human bone marrow CD34+ cells were fixed and used for ChIP and precipitated using 3 micrograms of antibody.

Sample: ChIP-seq analysis of N-Myc in human HSPCs, untreated, replicate 1

SAMN05560291 • SRS1610214 • All experiments • All runs

Organism: Homo sapiens

Library:

Instrument: Illumina HiSeq 2000

Strategy: ChIP-Seq

Source: GENOMIC

Selection: ChIP

Layout: SINGLE

Construction protocol: Thirty million human bone marrow CD34+ cells were fixed and used for ChIP and precipitated using 3 micrograms of antibody. Libraries were generated as described before, including end-repair, A-tailing, adapter (Illumina Truseq system) ligation and PCR amplification.

Experiment attributes:

GEO Accession: GSM2267690

Links:

NCBI link: NCBI Entrez (gds)

Runs: 1 run, 8.9M spots, 654.3M bases, 258.2Mb

Run	# of Spots	# of Bases	Size	Published
SRR4019569	8,861,539	654.3M	258.2Mb	2016-08-19

ID:: 2909983

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