GSM2185699: Ileum GFP+ Rep 2; Mus musculus; RNA-Seq - SRA

SRX1817250: GSM2185699: Ileum GFP+ Rep 2; Mus musculus; RNA-Seq
1 ILLUMINA (Illumina HiSeq 2000) run: 22.9M spots, 1.1G bases, 745.6Mb downloads

Submitted by: NCBI (GEO)

Study: Genome-wide identification of transcription factor ATOH1 target genes in adult small intestine and colon.

PRJNA324264 • SRP076034 • All experiments • All runs

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We generated three mRNA expression profiles by RNA-Seq of (i) wild-type crypts, (ii) Atoh1 knockout crypts, and (iii) purified ATOH1-positive cells. Overall design: We isolated Atoh1 knockout and littermate wild-type crypts from 6-8 week old Atoh1lox/lox;VilCreERT2 and Atoh1lox/WT;VilCreERT2 mice, respectively. In addition, ATOH1-positive cells were isolated by flow cytometry of 7AAD-negative (live), GFP-positive cells from either ileal or colonic crypts of Atoh1GFP/GFp mice (which express a functional ATOH1::GFP protein and are phenotypically normal).

Sample: Ileum GFP+ Rep 2

SAMN05199389 • SRS1479993 • All experiments • All runs

Organism: Mus musculus

Library:

Instrument: Illumina HiSeq 2000

Strategy: RNA-Seq

Source: TRANSCRIPTOMIC

Selection: cDNA

Layout: SINGLE

Construction protocol: RNA was harvested using Trizol reagent followed by RNeasy Kit (QUIGEN) RNA libraries were prepared for sequencing using standard Illumina protocols

Experiment attributes:

GEO Accession: GSM2185699

Links:

NCBI link: NCBI Entrez (gds)

Runs: 1 run, 22.9M spots, 1.1G bases, 745.6Mb

Run	# of Spots	# of Bases	Size	Published
SRR3621869	22,908,007	1.1G	745.6Mb	2017-01-05

ID:: 2598113

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