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SRX1713582: GSM2127460: H3K4me3 DMSO Be(2)-C; Homo sapiens; ChIP-Seq
1 ILLUMINA (Illumina HiSeq 2000) run: 22.7M spots, 1.2G bases, 484.1Mb downloads

Submitted by: NCBI (GEO)
Study: ChIP-seq-based analysis of differential K27me3 coverage in neuroblastoma cell lines treated with epigenetic drugs
PRJNA318912 • SRP073506 • All experiments • All runs
show Abstracthide Abstract
The impact of drugs inhibiting DNA methylation (5-aza-2''-deoxycytodine, DAC) and EZH2 (EPZ-6438) on H3K27me3 coverage was analyzed in two neuroblastoma cell lines. Parallel analyses investigated associated changes in RNA expression and DNA methylation. Overall design: The neuroblastoma cell lines Be(2)-C and IMR5-75 were treated with a combination of DAC and EPZ-6438. Controls were treated with solvent (DMSO). H3K27me3 ChIP seq was done to investigate treatment-related changes of this mark. In addition, H3K4me3 and H3K27ac ChIP seq was done in DMSO treated samples to identify putative regulatory regions.
Sample: H3K4me3 DMSO Be(2)-C
SAMN04870066 • SRS1402896 • All experiments • All runs
Organism: Homo sapiens
Library:
Instrument: Illumina HiSeq 2000
Strategy: ChIP-Seq
Source: GENOMIC
Selection: ChIP
Layout: SINGLE
Construction protocol: Lysates were clarified from sonicated nuclei and histone-DNA complexes were isolated with antibodies. Antibodies against H3K27ac (ab4729), H3K4me3 (ab8580, Abcam) and H3K27me3 (ab6002, Abcam) were used for precipitation. Library preparation was performed using the NEBNext Ultra DNA Library Prep Kit for Illumina (New England BioLabs, Ipswich, MA, US) according to the manufacturer’s protocol. Libraries were sequenced (50 bases single-end) on the Illumina sequencing platform (German Cancer Research Center Core facility).
Experiment attributes:
GEO Accession: GSM2127460
Links:
Runs: 1 run, 22.7M spots, 1.2G bases, 484.1Mb
Run# of Spots# of BasesSizePublished
SRR340284322,668,2481.2G484.1Mb2016-09-08

ID:
2454433

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