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SRX1561066: GSM2054775: antiH3K27cg-1-I; Drosophila melanogaster; ChIP-Seq
1 ILLUMINA (Illumina HiSeq 2500) run: 24.8M spots, 1.3G bases, 783.2Mb downloads

Submitted by: NCBI (GEO)
Study: Pho and Ph, H3K27me3 and Cg ChIP-seq data in Drosophila third instar larval brains and discs
show Abstracthide Abstract
Genome-wide binding profile of PcG proteins- Ph, H3K27me3 and Cg in Drosophila third instar larval brains and disks, in duplicate, using Illumina HiSeq 2500. The sequence reads that passed quality filters were analyzed. Overall design: Drosophila third instar larval brains and discs were dissected and fixed with 2% HCHO before sonication. After sonication, chromatin immunoprecipitation was carried out.
Sample: antiH3K27cg-1-I
SAMN04456655 • SRS1275515 • All experiments • All runs
Instrument: Illumina HiSeq 2500
Strategy: ChIP-Seq
Selection: ChIP
Layout: SINGLE
Construction protocol: Fixed brains and imaginal discs were dissected from 10 third instar larvae, and sonicated chromatin incubated with anti ChIP was performed with 1:100 dilutions of anti-Cg [custom made], anti-Ph (a kind gift from Donna Arndt-Jovin) antibodies and 1:200 dilutions of anti-H3K27me3 (Millipore, 17-622) antibodies. Input ChIP DNA (30 ng) is blunt-ended and phosphorylated. A single 'A' nucleotide is added to the 3' ends of the fragments in preparation for ligation to an adapter that has a single-base 'T' overhang. The ligation products are purified and accurately size-selected by agarose gel electrophoresis. Size-selected DNA is purified and PCR-amplified to enrich for fragments that have adapters on both ends. The final purified product is then quantitated before cluster generation.
Experiment attributes:
GEO Accession: GSM2054775
Runs: 1 run, 24.8M spots, 1.3G bases, 783.2Mb
Run# of Spots# of BasesSizePublished


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