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SRX1531769: GSM2040022: antiEnry_2; Drosophila melanogaster; ChIP-Seq
1 ILLUMINA (Illumina HiSeq 2500) run: 4.7M spots, 239.1M bases, 161.5Mb downloads

Submitted by: NCBI (GEO)
Study: Pho and Ph, H3K27me3 and Engrailed ChIP-seq data in Drosophila third instar larval brains and disks
show Abstracthide Abstract
Genome-wide binding profile of PcG proteins Pho and Ph, H3K27me3 and Engrailed in Drosophila third instar larval brains and disks, in duplicate, using Illumina HiSeq 2500. The sequence reads that passed quality filters were analyzed. Overall design: Drosophila third instar larval brains and disks were dissected and fixed with 2% HCHO before sonication. After sonication, chromatin immunoprecipitation using anti-Pho, -Ph, -H3K27me3, and -Engrailed antibodies was carried out.
Sample: antiEnry_2
SAMN04415431 • SRS1249084 • All experiments • All runs
Instrument: Illumina HiSeq 2500
Strategy: ChIP-Seq
Selection: ChIP
Layout: SINGLE
Construction protocol: Fixed brains and imaginal discs were dissected from 10 third instar larvae. Sonicated chromatin incubated with anti ChIP was performed with 1:100 dilutions of anti-Pho (custom made) and anti-Ph antibodies (a kind gift from Donna Arndt-Jovin), 1:200 dilutions of anti-H3K27me3 (Millipore, 17-622) antibodies, and anti-Engrailed antibodies. Input ChIP DNA (30 ng) was blunt-ended and phosphorylated. A single 'A' nucleotide was added to the 3' ends of the fragments in preparation for ligation to an adapter that has a single-base 'T' overhang. The ligation products were purified and accurately size-selected by agarose gel electrophoresis. Size-selected DNA was purified and PCR-amplified to enrich for fragments that have adapters on both ends. The final purified product was then quantitated before cluster generation.
Experiment attributes:
GEO Accession: GSM2040022
Runs: 1 run, 4.7M spots, 239.1M bases, 161.5Mb
Run# of Spots# of BasesSizePublished


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