GSM1669062: JC1380_MCF7_p300_R5020_3hr r0; Homo sapiens; ChIP-Seq - SRA

SRX1012645: GSM1669062: JC1380_MCF7_p300_R5020_3hr r0; Homo sapiens; ChIP-Seq
2 ILLUMINA (Illumina HiSeq 2000) runs: 14.7M spots, 530.6M bases, 660Mb downloads

Submitted by: Gene Expression Omnibus (GEO)

Study: ChIP-seq of ER, PR, p300 in two ER+ breast cancer cell lines treated with estrogen and with and without progestins

PRJNA282508 • SRP057755 • All experiments • All runs

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Exploring effect of progesterone/progestin treatment on ER and PR binding. Overall design: Two cell lines, three conditions (Full Media with E2, E2+ Progesterone, Full Media + R5020 Progestin), three factors (ER, PR, p300), all with three replicates, each with a matched Input control.

Sample: JC1380_MCF7_p300_R5020_3hr r0

SAMN03570672 • SRS924402 • All experiments • All runs

Organism: Homo sapiens

Library:

Instrument: Illumina HiSeq 2000

Strategy: ChIP-Seq

Source: GENOMIC

Selection: ChIP

Layout: SINGLE

Construction protocol: Immunoprecipitated DNA was end-repaired, A-tailed, ligated to the sequencing adapters, amplified by 18 cycles of PCR and size selected (200-300 bp) followed by single end 50bp sequencing on an Illumina HiSeq 2000 according to the manufacturer’s recommendations

Experiment attributes:

GEO Accession: GSM1669062

Links:

External link: GEO Sample GSM1669062

NCBI link: NCBI Entrez (gds)

Runs: 2 runs, 14.7M spots, 530.6M bases, 660Mb

Run	# of Spots	# of Bases	Size	Published
SRR2000769	7,364,809	265.1M	331.6Mb	2015-05-22
SRR2000770	7,373,016	265.4M	328.4Mb	2015-05-22

ID:: 1467497

SRA

Sequence Read Archive

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