GSM1669043: JC1533_MCF7_ER_Full_Media_3hr r2; Homo sapiens; ChIP-Seq - SRA

SRX1012626: GSM1669043: JC1533_MCF7_ER_Full_Media_3hr r2; Homo sapiens; ChIP-Seq
2 ILLUMINA (Illumina HiSeq 2000) runs: 30.1M spots, 1.2G bases, 716.9Mb downloads

Submitted by: Gene Expression Omnibus (GEO)

Study: ChIP-seq of ER, PR, p300 in two ER+ breast cancer cell lines treated with estrogen and with and without progestins

PRJNA282508 • SRP057755 • All experiments • All runs

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Exploring effect of progesterone/progestin treatment on ER and PR binding. Overall design: Two cell lines, three conditions (Full Media with E2, E2+ Progesterone, Full Media + R5020 Progestin), three factors (ER, PR, p300), all with three replicates, each with a matched Input control.

Sample: JC1533_MCF7_ER_Full_Media_3hr r2

SAMN03570651 • SRS924397 • All experiments • All runs

Organism: Homo sapiens

Library:

Instrument: Illumina HiSeq 2000

Strategy: ChIP-Seq

Source: GENOMIC

Selection: ChIP

Layout: SINGLE

Construction protocol: Immunoprecipitated DNA was end-repaired, A-tailed, ligated to the sequencing adapters, amplified by 18 cycles of PCR and size selected (200-300 bp) followed by single end 50bp sequencing on an Illumina HiSeq 2000 according to the manufacturer’s recommendations

Experiment attributes:

GEO Accession: GSM1669043

Links:

External link: GEO Sample GSM1669043

NCBI link: NCBI Entrez (gds)

Runs: 2 runs, 30.1M spots, 1.2G bases, 716.9Mb

Run	# of Spots	# of Bases	Size	Published
SRR2000734	15,083,173	603.3M	360.1Mb	2015-05-22
SRR2000735	15,049,045	602M	356.8Mb	2015-05-22

ID:: 1467478

SRA

Sequence Read Archive

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