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SRX040992: ChIP-Seq analysis of H3K27me3 in human Mobilized CD34 cells
1 ILLUMINA (Illumina Genome Analyzer IIx) run: 24.7M spots, 888.6M bases, 650.2Mb downloads

Design: Single read - Illumina
Submitted by: Gene Expression Omnibus (GEO)
Study: BI Human Reference Epigenome Mapping Project: ChIP-Seq in human subject
show Abstracthide Abstract
Characterization of the reference epigenome in humans by use of ChIP-Seq in a diverse panel of ES cells, tissue stem cells, reprogrammed stem cells, primary cells and tissues.
Sample: Generic sample from Homo sapiens
SAMN00119945 • SRS120868 • All experiments • All runs
Organism: Homo sapiens
Library:
Name: Lib:RI:20100308:09--ChIP:MA:20100216:09:Mobilized CD34:H3K27Me3
Instrument: Illumina Genome Analyzer IIx
Strategy: ChIP-Seq
Source: GENOMIC
Selection: ChIP
Layout: SINGLE
Spot descriptor:
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Experiment attributes: (show all 14 attributes...) (hide...)
CHIP_PROTOCOL_BEAD_TYPE: Protein A-Sepharose 4B
CHIP_ANTIBODY: H3K27Me3
CHIP_PROTOCOL_CHROMATIN_AMOUNT: 10 million cells
EXTRACTION_PROTOCOL: SDS lysis
CHIP_PROTOCOL_ANTIBODY_AMOUNT: 2-15 ug
CHIP_PROTOCOL_BEAD_AMOUNT: 70 ul bed volume
CHIP_PROTOCOL: Bernstein_BROAD_ENCODE_protocol
EXTRACTION_PROTOCOL_SONICATION_CYCLES: 1
CHIP_ANTIBODY_CATALOG: 07-449
CHIP_ANTIBODY_PROVIDER: Upstate
CHIP_ANTIBODY_LOT: dam151401
EXPERIMENT_TYPE: Histone H3K27me3
EXTRACTION_PROTOCOL_TYPE_OF_SONICATOR: Bioruptor
GEO Accession: GSM669945
Pipeline: show...hide...
NameStepPrev StepProgramVersion
BASE_CALLS1NILGAPipeline1.6.1
QUALITY_SCORES21GAPipeline1.6.1
Links:
External link:
External link:
Runs: 1 run, 24.7M spots, 888.6M bases, 650.2Mb
Run# of Spots# of BasesSizePublished
SRR106751924,683,312888.6M650.2Mb2014-01-03

ID:
48441

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