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SRX030163: C. thermocellum ATCC 27405 Resequencing
3 LS454 (454 GS FLX) runs: 946,851 spots, 255.2M bases, 512.1Mb downloads

Design: Constructed following GS FLX Shotgun DNA Library Prepatation Method Manual following steps for high molecular weight samples (using nebulization for fragmentation).
Submitted by: Oak Ridge National Laboratory
Study: Mutant alcohol dehydrogenase leads to improved ethanol tolerance in Clostridium thermocellum
show Abstracthide Abstract
Clostridium thermocellum is a thermophilic, obligately anaerobic, gram-positive bacterium that is a candidate microorganism for converting cellulosic biomass into ethanol through consolidated bioprocessing. Ethanol intolerance is an important metric in terms of process economics, and tolerance has often been described as a complex and likely multigenic trait for which complex gene interactions come into play. Here, we resequence the genome of an ethanol-tolerant mutant, show that the tolerant phenotype is primarily due to a mutated bifunctional acetaldehyde-CoA/alcohol dehydrogenase gene (adhE), hypothesize based on structural analysis that cofactor specificity may be affected, and confirm this hypothesis using enzyme assays. Biochemical assays confirm a complete loss of NADH-dependent activity with concomitant acquisition of NADPH-dependent activity, which likely affects electron flow in the mutant. The simplicity of the genetic basis for the ethanol-tolerant phenotype observed here informs rational engineering of mutant microbial strains for cellulosic ethanol production.
Sample: C. thermocellum ATCC 27405
SAMN00014193 • SRS074210 • All experiments • All runs
Name: Bulk
Instrument: 454 GS FLX
Strategy: WGS
Selection: RANDOM
Layout: SINGLE
Spot descriptor:

Runs: 3 runs, 946,851 spots, 255.2M bases, 512.1Mb
Run# of Spots# of BasesSizePublished


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