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SRX1670049: Multi-omics of human faecal sample: metagenomics
1 ILLUMINA (Illumina HiSeq 2000) run: 23.8M spots, 4G bases, 2.4Gb downloads

Design: Libraries with an insert size of 350 bp were constructed from metagenomic DNA by fragmentation by sonification (Covaris), end-repair, adenylation, adapter ligation and amplifaction of adapter-ligated DNA fragments using appropriate enzymes (Enzymatics). Library amplification and cluster generation were performed using TruSeq PE Cluster Kit V3-cBot-HS (Illumina) and flow cells were sequenced with 101 bp per paired end on a HiSeq2000 system (Illumina).
Submitted by: Luxembourg Centre for Systems Biomedicine
Study: Human Gut Microbiome in a Multiplex Family Study of Type 1 Diabetes Mellitus
show Abstracthide Abstract
Recent evidence from high-resolution molecular studies suggests links between microbial dysbiosis in the gastrointestinal tract and a number of complex chronic diseases including diabetes mellitus. The present study forms a pilot study for the larger Luxembourg-based Diabetes Multiplex Family Study (MUST). Within the pilot study, we investigate the interplay of the gastrointestinal microbiota, lifestyle and genetic background in an observational study of families with two or more individuals affected by type 1 diabetes mellitus. Faecal samples which have undergone comprehensive biomolecular extractions are currently analysed using a multi-omics approach. Anthropometric data, including demographics, medical history, health status, medication, and dietary habits of all study participants were collected and are integrated with molecular data.
Sample:
SAMN04571721 • SRS1369976 • All experiments • All runs
Library:
Name: M03.5-V2-stool-metaG
Instrument: Illumina HiSeq 2000
Strategy: WGS
Source: METAGENOMIC
Selection: RANDOM
Layout: PAIRED
Runs: 1 run, 23.8M spots, 4G bases, 2.4Gb
Run# of Spots# of BasesSizePublished
SRR331306523,782,9724G2.4Gb2016-08-12

ID:
2395943

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