RNA-Seq fom meiocytes of the barley cultivar Golden Promise - SRA

SRX6642413: RNA-Seq fom meiocytes of the barley cultivar Golden Promise
1 ILLUMINA (NextSeq 550) run: 87.9M spots, 13.3G bases, 4.9Gb downloads

Design: Around 30-50 anthers of 1.0 1.2 mm size were collected. The tips of the anthers were cut with insulin needles and were tapped gently to release the meiocytes bag. Using RNase-free 10 l tips, released meiocytes were transferred into a new 0.5 ml Eppendorf tube containing 10 l of citrate buffer and anther tissue was discarded. RNA was extracted using TRIzol PlusRNA Purification Kit (Thermofisher Scientific) following the manufacturers instructions). RNA-seq libraries were constructed using TruSeq mRNA Sample Preparation kit (Illumina) as recommended by the manufacturer. Libraries were quality checked using a Bioanalyzer 2100 (Agilent) and quantified by both qPCR (KAPA Library Quantification Kit, KAPA Biosystems) and using a Qubit fluorometer.

Submitted by: The James Hutton Institute

Study: Hordeum vulgare anther and meiocyte transcriptome

PRJNA558196 • SRP217154 • All experiments • All runs

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We performed Illumina sequencing of cytologically staged isolated barley anthers at four differend timepoints and purified meiocytes and two different timepoints. We also included three IsoSeq PacBio libraries from a mixture of the above stages.

Sample:

SAMN12429992 • SRS5199449 • All experiments • All runs

Organism: Hordeum vulgare

Library:

Name: M-PachDipl1

Instrument: NextSeq 550

Strategy: RNA-Seq

Source: TRANSCRIPTOMIC

Selection: cDNA

Layout: PAIRED

Runs: 1 run, 87.9M spots, 13.3G bases, 4.9Gb

Run	# of Spots	# of Bases	Size	Published
SRR9889997	87,874,221	13.3G	4.9Gb	2020-03-19

ID:: 8763046

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